![]() In this work, with two concrete cases (WuXiBodies with asymmetric and symmetric designs, respectively), we showed that Capto MMC ImpRes mixed-mode chromatography can effectively remove LC-missing byproducts as well as aggregates, demonstrating that this resin is a powerful tool for WuXiBody purification.Īntibody aggregates Bispecific antibody (bsAb) Capto MMC ImpRes Light chain (LC)-missing byproduct Mixed-mode chromatography WuXiBody.Ĭopyright © 2020 Elsevier Inc. scales by Ian Ring capto mmc impres scle finder ian ring mean Scale. Nevertheless, WuXiBody's unique design greatly facilitates removal of this type of impurity, which can otherwise be difficult to clear. in the scale that does not have the capto mmc compression factor Finder. ![]() LC-missing species, a byproduct frequently associated with bsAb containing sequence engineered Fab arm, is also identified during WuXiBody production. Each bead has a ligand-activated core and an outer layer without ligands. Ho eseguito una Scintigrafia miocardica al tallio da sforzo e a riposo mediante spect. This chromatography resin is part of an expanded high-resolution platform based on the high-flow agarose Capto product line. The product is based on the core bead concept. Capto SP ImpRes is a strong cation exchanger for the high-throughput intermediate purification and polishing steps of a wide range of biomolecules. BsAbs based on WuXiBody can adopt either asymmetric or symmetric format. CaptoTM Core 700 chromatography medium is aimed at intermediate purification and polishing of viruses and other large biomolecules in flow- through mode. Properties ligand sulfonate group description Ion Exchanger Type (value) shelf life Please be aware this product may be shipped 90 days before the expiration date. Its key feature is the replacement of one parental antibody's CH1/CL constant region with the T cell receptor (TCR) constant domain, a design aimed at promoting cognate heavy chain (HC)-light chain (LC) pairing. Capto SP ImpRes Cytiva 17-5468-02, pack of 100 mL NACRES: NA.56 Pricing and availability is not currently available. In this work, we evaluated this resin using Cytivas Capto MMC ImpRes, a well-established mixed-mode resin with similar properties, as a benchmark. ![]() However, the dynamic binding capacity (DBC) of Capto MMC ImpRes is relatively low under the selected condition, making the process less desirable for large-scale manufacturing. WuXiBody is a novel bispecific antibody (bsAb) platform developed by WuXi Biologics. Diamond MMC Mustang is a relatively new mixed-mode resin, which mediates both cation exchange and hydrophobic interactions. We previously showed that Capto MMC ImpRes mixed-mode chromatography under bind-elute mode can effectively remove this LC-missing species. ![]()
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